ó㳺í³÷íà îö³íêà ³ìóíîòîêñè÷íèõ åôåêò³â çà êîìá³íîâàíî¿ ä³¿ ïð³îðèòåòíèõ çàáðóäíåíü âîäíîãî ñåðåäîâèùà

19. Äåêëàðàö³éíèé ïàòåíò íà êîðèñíó ìîäåëü 7252 Óêðà¿íà G01N33/53. Ñïîñ³á âèçíà÷åííÿ ³ìóííî¿ â³äïîâ³ä³ îðãàí³çìó íà êîìá³íîâàíó ä³þ êñåíîá³îòèê³â äîâê³ëëÿ / Î.². Âèíàðñüêà, Í.Î. ͳêîíîâà, Ë.ª. Ãðèãîðåíêî, Ñ.Â. Ëóê'ÿí÷óê; çàÿâíèê ³ ïàòåíòîâëàñíèê ÄÓ „²ÃÌÅ ÀÌÍÓ”. - ¹ 20041109265; çàÿâë. 12.11.2004; îïóáë. 15.06.2005, Áþë. ¹ 6. (Äèñåðòàíòîì ïðîâåäåíî ïàòåíòíèé ïîøóê, äîñë³äæåííÿ, îôîðìëåíî ïàòåíò).

20. Äåêëàðàö³éíèé ïàòåíò íà êîðèñíó ìîäåëü 31951 Óêðà¿íà ÌÏÊ⁵¹ G01N 33/53. Ñïîñ³á âèçíà÷åííÿ àäàïòàö³éíî-ïðèñòîñîâíèõ ìîæëèâîñòåé îðãàí³çìó çà 䳿 íèçüêèõ ð³âí³â øê³äëèâèõ ÷èííèê³â äîâê³ëëÿ / Î.². Âèíàðñüêà, ².Â. Êîíîíêî, Ë.ª. Ãðèãîðåíêî, Ñ.Â. Ëóê'ÿí÷óê; çàÿâíèê ³ ïàòåíòîâëàñíèê ÄÓ „²ÃÌÅ ÀÌÍÓ”.-¹ 200714719 çàÿâë. 25.12.2007; îïóáë. 25.04.2008, Áþë. ¹ 8. (Äèñåðòàíòîì ïðîâåäåíî ïàòåíòíèé ïîøóê, åêñïåðèìåíòàëüí³ äîñë³äæåííÿ, îôîðìëåíî ïàòåíò).

21. ²íôîðìàö³éíèé ëèñò ¹ 178-2007 „Ïàðàìåòðè ³ìóííîãî ñòàòóñó ëàáîðàòîðíèõ òâàðèí (â³êîâ³ íîðìè)” / Î.². Âèíàðñüêà, Ñ.Â. Ëóê'ÿí÷óê, ².Â. Êîíîíêî, Ë.ª. Ãðèãîðåíêî. - Ê.: Óêðìåäïàòåíò³íôîðì, 2007 ð. - 3 ñ. (Çäîáóâà÷åì ïðîâåäåíî äîñë³äæåííÿ, îôîðìëåíî ³íôîðìàö³éíèé ëèñò).

22. ²íôîðìàö³éíèé ëèñò ¹ 118-2009 „Îö³íêà çíà÷óùîñò³ çì³í â ³ìóíí³é ñèñòåì³ çà óìîâè êîìá³íîâàíî¿ ä³¿ óá³êâ³òàðíèõ êñåíîá³îòèê³â âîäíîãî ñåðåäîâèùà” / Î.². Âèíàðñüêà, Ñ.Â. Ëóê'ÿí÷óê, Ë.ª. Ãðèãîðåíêî, Ò.À. ×óáóê. - Ê.: Óêðìåäïàòåíò³íôîðì, 2009 ð. - 3 ñ. (Çäîáóâà÷åì ïðîâåäåíî åêñïåðèìåíòàëüí³ äîñë³äæåííÿ, îôîðìëåíî ³íôîðìàö³éíèé ëèñò).

ÀÍÎÒÀÖ²ß

Ëóê'ÿí÷óê Ñ.Â. “ó㳺í³÷íà îö³íêà ³ìóíîòîêñè÷íèõ åôåêò³â çà êîìá³íîâàíî¿ ä³¿ ïð³îðèòåòíèõ çàáðóäíåíü âîäíîãî ñåðåäîâèùà” - Ðóêîïèñ.

Äèñåðòàö³ÿ íà çäîáóòòÿ â÷åíîãî ñòóïåíÿ êàíäèäàòà á³îëîã³÷íèõ íàóê ç³ ñïåö³àëüíîñò³ 14.02.01 - “ã³ã³ºíà òà ïðîôåñ³éíà ïàòîëîã³ÿ” (á³îëîã³÷í³ íàóêè) - Äåðæàâíà óñòàíîâà “²íñòèòóò ã³ã³ºíè òà ìåäè÷íî¿ åêîëî㳿 ³ì. Î.Ì. Ìàð纺âà ÀÌÍ Óêðà¿íè”, Êè¿â, 2010.

Äèñåðòàö³ÿ ïðèñâÿ÷åíà îáãðóíòóâàííþ çíà÷óùîñò³ ³ìóíîòîêñè÷íèõ åôåêò³â äëÿ ã³ã³ºí³÷íî¿ îö³íêè êîìá³íîâàíî¿ ä³¿ ïð³îðèòåòíèõ çàáðóäíåíü âîäíîãî ñåðåäîâèùà.  åêñïåðèìåíòàëüíèõ äîñë³äæåííÿõ íà á³ëèõ áåçïîð³äíèõ ùóðàõ âèâ÷àëè çàêîíîì³ðíîñò³ êîìá³íîâàíîãî âïëèâó ð³çíèõ äîç ôåíîëó òà õëîðîôîðìó, à òàêîæ ôåíîëó, í³òðèòó íàòð³þ ³ í³òðàòó íàòð³þ íà ³ìóííó ñèñòåìó çà ïåðîðàëüíîãî íàäõîäæåííÿ â îðãàí³çì ç ïèòíîþ âîäîþ ïðîòÿãîì 6 ì³ñÿö³â. Âèÿâëåíî îñîáëèâîñò³ òà õàðàêòåð êîìá³íîâàíîãî âïëèâó ôåíîëó é õëîðîôîðìó, à òàêîæ ôåíîëó, í³òðèòó íàòð³þ òà í³òðàòó íàòð³þ íà ³ìóííó ñèñòåìó îðãàí³çìó; âñòàíîâëåíî çàêîíîì³ðíîñò³ ðîçâèòêó ñåíñèá³ë³çóþ÷îãî òà ³ìóíîìîäóëþþ÷îãî åôåêò³â êîìá³íîâàíîãî âïëèâó äîñë³äæóâàíèõ ñïîëóê â çàëåæíîñò³ â³ä äîçè òà òðèâàëîñò³ 䳿; âñòàíîâëåíî çíà÷óù³ñòü äëÿ îðãàí³çìó çì³í â ³ìóíí³é ñèñòåì³ çà êîìá³íîâàíîãî âïëèâó ôåíîëó é õëîðîôîðìó; ôåíîëó òà í³òðèòó íàòð³þ ³ í³òðàòó íàòð³þ.

Êëþ÷îâ³ ñëîâà: ³ìóííà ñèñòåìà, êîìá³íîâàíèé âïëèâ, ôåíîë, õëîðîôîðì, í³òðèò íàòð³þ, í³òðàò íàòð³þ, åêñïåðèìåíòàëüí³ òâàðèíè.

Ëóêüÿí÷óê Ñ.Â. “Ãèãèåíè÷åñêàÿ îöåíêà èììóíîòîêñè÷åñêèõ ýôôåêòîâ ïðè êîìáèíèðîâàííîì âîçäåéñòâèè ïðèîðèòåòíûõ çàãðÿçíåíèé âîäíîé ñðåäû” - Ðóêîïèñü.

Äèñcåðòàöèÿ íà ñîèñêàíèå ó÷åíîé ñòåïåíè êàíäèäàòà áèîëîãè÷åñêèõ íàóê ïî ñïåöèàëüíîñòè 14.02.01 - “ãèãèåíà è ïðîôåññèîíàëüíàÿ ïàòîëîãèÿ” (áèîëîãè÷åñêèå íàóêè) - Ãîñóäàðñòâåííîå ó÷ðåæäåíèå “Èíñòèòóò ãèãèåíû è ìåäèöèíñêîé ýêîëîãèè èì. À.Í. Ìàðçååâà ÀÌÍ Óêðàèíû”, Êèåâ, 2010.

Äèññåðòàöèÿ ïîñâåùåíà îáîñíîâàíèþ çíà÷èìîñòè èììóíîòîêñè÷åñêèõ ýôôåêòîâ äëÿ ãèãèåíè÷åñêîé îöåíêè êîìáèíèðîâàííîãî äåéñòâèÿ ïðèîðèòåòíûõ çàãðÿçíåíèé âîäíîé ñðåäû.  ýêñïåðèìåíòàëüíûõ èññëåäîâàíèÿõ íà áåëûõ áåñïîðîäíûõ êðûñàõ èçó÷àëè çàêîíîìåðíîñòè êîìáèíèðîâàííîãî âëèÿíèÿ ðàçëè÷ííûõ äîç ôåíîëà è õëîðîôîðìà, à òàêæå ôåíîëà, íèòðèòà íàòðèÿ è íèòðàòà íàòðèÿ íà èììóííóþ ñèñòåìó ïðè ïåðîðàëüíîì ïîñòóïëåíèè â îðãàíèçì ñ ïèòüåâîé âîäîé â òå÷åíèå 6 ìåñÿöåâ.

Íà îñíîâàíèè ïðîâåäåííûõ èññëåäîâàíèé îïðåäåëåíû îñîáåííîñòè è õàðàêòåð êîìáèíèðîâàííîãî âîçäåéñòâèÿ õëîðîôîðìà è ôåíîëà; íèòðèòà íàòðèÿ, íèòðàòà íàòðèÿ è ôåíîëà íà èììóííóþ ñèñòåìó îðãàíèçìà; óñòàíîâëåíû çàêîíîìåðíîñòè ñåíñèáèëèçèðóþùåãî è èììóíîìîäóëèðóþùåãî ýôôåêòîâ êîìáèíèðîâàííîãî âëèÿíèÿ èññëåäóåìûõ ñîåäèíåíèé îò äîçû è ñðîêà âîçäåéñòâèÿ; óñòàíîâëåíà çíà÷èìîñòü äëÿ îðãàíèçìà èçìåíåíèé â èììóííîé ñèñòåìå ïðè êîìáèíèðîâàííîì âîçäåéñòâèè õëîðîôîðìà è ôåíîëà, à òàêæå íèòðèòà íàòðèÿ, íèòðàòà íàòðèÿ è ôåíîëà. ³ìóííèé õëîðîôîðì ôåíîë õ³ì³÷íèé

Óñòàíîâëåíî, ÷òî ðèñê ðàçâèòèÿ èììóíîëîãè÷åñêèõ ýôôåêòîâ îïðåäåëÿåòñÿ ðåàêöèåé èììóííîé ñèñòåìû íà àíòèãåííóþ íàãðóçêó, êîòîðàÿ ïîçâîëÿåò âûÿâèòü êîìïåíñàòîðíî-ïðèñïîñîáèòåëüíûå âîçìîæíîñòè îðãàíèçìà. Èñïîëüçîâàíèå íàãðóçî÷íîãî òåñòà ïîêàçàëî ñòîéêîñòü èçìåíåíèé â èììóííîé ñèñòåìå æèâîòíûõ, êîòîðûå ïîäâåðãàëèñü êîìáèíèðîâàííîìó âîçäåéñòâèþ êñåíîáèîòèêîâ. Õàðàêòåð íàðóøåíèé çàâèñåë îò êà÷åñòâåííîãî ñîñòàâà ñìåñè è äîçû êñåíîáèîòèêîâ. Ïðè êîìáèíèðîâàííîì âëèÿíèè ôåíîëà è õëîðîôîðìà íà óðîâíÿõ ãèãèåíè÷åñêèõ íîðìàòèâîâ íàáëþäàëàñü ãèïåð÷óâñòâèòåëüíîñòü íåìåäëåííîãî òèïà, èììóíîñóïðåññèÿ ïî Â-êëåòî÷íîìó òèïó, à ïðè ïîâûøåíèè êîíöåíòðàöèé õèìè÷åñêèé ñîåäèíåíèé â 3 è 9 ðàç - óãíåòåíèå êàê êëåòî÷íîãî, òàê è ãóìîðàëüíîãî çâåíüåâ èììóííîé ñèñòåìû íà ôîíå ñåíñèáèëèçàöèè è àóòîñåíñèáèëèçàöèè îðãàíèçìà. Ïðè êîìáèíèðîâàííîì âîçäåéñòâèè íèòðèòà íàòðèÿ (16,5 ìã/äì³), íèòðàòà íàòðèÿ (225 ìã/äì³) è ôåíîëà (0,003 ìã/äì³) íàáëþäàëèñü èçìåíåíèÿ â êëåòî÷íîì çâåíà èììóíèòåòà.

Ïîêàçàíî, ÷òî ðàííèìè è ñïåöèôè÷åñêèìè ýôôåêòàìè ïðè êîìáèíèðîâàííîì äåéñòâèè íèòðèòà íàòðèÿ, íèòðàòà íàòðèÿ è ôåíîëà â çàâèñèìîñòè îò äîç êñåíîáèîòèêîâ ÿâëÿþòñÿ èììóíîñóïðåññèÿ ïî Ò- è Â-êëåòî÷íîìó òèïó è ðàçâèòèå ãèïåð÷óâñòâèòåëüíîñòè íåìåäëåííîãî òèïà. Õàðàêòåð è ñïåêòð èçìåíåíèé çàâèñåëè îò äîçû õèìè÷åñêîãî ñîåäèíåíèÿ.

Âûÿâëåíà ìîäóëÿöèÿ õàðàêòåðà ýôôåêòîâ ïðè êîìáèíèðîâàííîì äåéñòâèè ôåíîëà, íèòðèòà íàòðèÿ è íèòðàòà íàòðèÿ, êîòîðàÿ ïðîÿâëÿëàñü ôàçíîñòüþ ðàçâèòèÿ ÃÍÒ, íàðóøåíèé â êëåòî÷íîì è ãóìîðàëüíîì çâåíüÿõ èììóííîé ñèñòåìû, íåñïåöèôè÷åñêèõ ôàêòîðîâ çàùèòû îðãàíèçìà. Âûðàæåíîñòü ýôôåêòîâ çàâèñåëà îò äîçû êñåíîáèîòèêîâ â ñìåñè è ñðîêà èõ âîçäåéñòâèÿ.

Òàêèì îáðàçîì, ðåçóëüòàòû ïðîâåäåííûõ ýêñïåðèìåíòàëüíûõ èññëåäîâàíèé ïîçâîëèëè óñòàíîâèòü îñíîâíûå çàêîíîìåðíîñòè ïðîÿâëåíèÿ èììóíîòîêñè÷åñêîãî äåéñòâèÿ õèìè÷åñêèõ çàãðÿçíèòåëåé âîäíîé ñðåäû. Êîìáèíèðîâàííîå âëèÿíèå ôåíîëà è õëîðîôîðìà, à òàêæå íèòðèòà íàòðèÿ, íèòðàòà íàòðèÿ è ôåíîëà ïðèâîäèò ê äîçî-âðåììåííîé ìîäóëÿöèè è ìîäèôèêàöèè èììóííîãî îòâåòà. Õàðàêòåð è âûðàæåííîñòü èììóíîòîêñè÷åñêèõ ýôôåêòîâ çàâèñÿò îò äîçû, ñðîêà âîçäåéñâòèÿ è êà÷åñòâåííîãî ñîñòàâà êîìáèíàöèé.

Êëþ÷åâûå ñëîâà: èììóííàÿ ñèñòåìà, êîìáèíèðîâàííîå äåéñòâèå, ôåíîë, õëîðîôîðì, íèòðèò íàòðèÿ, íèòðàò íàòðèÿ, ýêñïåðèìåíòàëüíûå æèâîòíûå.

Lukyanchuk S. ”Hygienic evaluation of immune toxic effects under combined exposure of priority water pollutants”. - Manuscript.

Dissertation is presented for Biological Candidate's Degree by the specialty 14.02.01 - “hygiene and occupational pathology” (biological sciences). - State Establishment “O.M. Marzeyev Institute of Hygiene and Medical Ecology of Academy of Medical Sciences of Ukraine”, Kyiv, 2010.

Dissertation is devoted to the ground of significance of immunotoxic effects for the hygienic evaluation of combined exposure of priority water pollutants. Conformations of combined exposure of phenol and chloroform; phenol, sodium nitrite and sodium nitrate for the immune system under per oral entering to organism of the different doses of chemical substances with drinking water during 6 months have been studied in the experimental researches on the white outbreed rats. The peculiarities and character of the combined exposure of phenol and chloroform; phenol, sodium nitrite and sodium nitrate for the organism immune system have been revealed. The conformations of development of sensitization and immune modullation of combined exposure of research substances have been determined in dependence on dose and duration of exposure. The significance of changes in immune system for organism under combined exposure of phenol and chloroform; phenol, sodium nitrite and sodium nitrate have been established.

Key words: immune system, combine exposure, phenol, chloroform, sodium nitrite, sodium nitrate, experimental animals.

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